Mechanism of Cav1.2 channel modulation by the amino terminus of cardiac 2-subunits

L-type calcium channels are composed of a pore, 1c (CaV1.2), and accessory and 2 subunits. The -subunit core structure was recently resolved at high resolution, providing important information on many functional aspects of channel modulation. In this study we reveal differential novel effects of five 2-subunits isoforms expressed in human heart ( 2a-e) on the single L-type calcium channel current. These splice variants differ only by amino-terminal length and amino acid composition. Single-channel modulation by 2-subunit isoforms was investigated in HEK293 cells expressing the recombinant L-type ion conducting pore. All 2-subunits increased open probability, availability, and peak current with a highly consistent rank order ( 2a 2b> 2e 2c> 2d). We show graded modulation of some transition rates within and between deep-closed and inactivated states. The extent of modulation correlates strongly with the length of amino-terminal domains. Two mutant 2-subunits that imitate the natural span related to length confirm this conclusion. The data show that the length of amino termini is a relevant physiological mechanism for channel closure and inactivation, and that natural alternative splicing exploits this principle for modulation of the gating properties of calcium channels.—Herzig, S., Khan, I. F. Y., Gründemann, D., Matthes, J., Ludwig, A., Michels, G., Hoppe, U. C., Chaudhuri, D., Schwartz, A., Yue, D. T., Hullin, R. Mechanism of Cav1.2 channel modulation by the amino terminus of cardiac 2-subunits. FASEB J. 21, 1527–1538 (2007)